Gibco™DMEM/F-12

Product Number : 11320033


Gibco™DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM/F-12 is modified as follows:


With
Without
L-glutamine
HEPES
Phenol Red


The complete formulation is available.


Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system

DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (11320-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.


사양


농도1X
제품 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12
멸균Sterile-filtered
혈청 보충제Standard Serum Supplementation
배송 조건Room Temperature
부피(미터법)500 ml
제품라인Gibco™
형태Liquid
글루타민L-Glutamine
페놀 레드 인디케이터Phenol Red
유통 기한12 Months From Date of Manufacture
그린 기능Sustainable packaging
수량500mL
셀 유형primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells
첨가제 포함L-Glutamine, Phenol Red
첨가제 없음HEPES
GlucoseHigh Glucose
Sodium Pyruvate AdditiveSodium Pyruvate
세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
분류Animal Origin-Free
용액 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12
HEPES BufferNo HEPES

구성 및 보관


  • Storage conditions: 2°C to 8°C (protect from light)
  • Shipping conditions: Ambient
  • Shelf life: 12 months from date of manufacture

배지 조성


  • 11320 - DMEM/F-12