Gibco™DMEM/F-12
Product Number : 11320033
Gibco™DMEM/F-12
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With | Without |
L-glutamine | HEPES |
Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (11320-074). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
사양
농도 | 1X |
제품 유형 | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
멸균 | Sterile-filtered |
혈청 보충제 | Standard Serum Supplementation |
배송 조건 | Room Temperature |
부피(미터법) | 500 ml |
제품라인 | Gibco™ |
형태 | Liquid |
글루타민 | L-Glutamine |
페놀 레드 인디케이터 | Phenol Red |
유통 기한 | 12 Months From Date of Manufacture |
그린 기능 | Sustainable packaging |
수량 | 500mL |
셀 유형 | primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells |
첨가제 포함 | L-Glutamine, Phenol Red |
첨가제 없음 | HEPES |
Glucose | High Glucose |
Sodium Pyruvate Additive | Sodium Pyruvate |
세포주 | MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts |
분류 | Animal Origin-Free |
용액 유형 | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
HEPES Buffer | No HEPES |
구성 및 보관
배지 조성