Gibco™ RPMI 1640 Medium

Product Number : 11875093


Gibco™ RPMI 1640 Medium

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This RPMI is modified as follows:


With
Without
L-glutamine
HEPES
Phenol Red


The complete formulation is available.


Using RPMI

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


Product use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).


cGMP manufacturing and quality system

RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-158). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


사양


농도1X
제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
멸균Sterile-filtered
혈청 보충제Standard Serum Supplementation
배송 조건Room Temperature
부피(미터법)500 ml
제품라인Gibco™
형태Liquid
글루타민L-Glutamine
페놀 레드 인디케이터Phenol Red
유통 기한12 Months From Date of Manufacture
그린 기능Sustainable packaging
수량500mL
셀 유형leukemic cells
첨가제 포함L-Glutamine, Phenol Red
첨가제 없음HEPES
Sodium Pyruvate AdditiveNo Sodium Pyruvate
세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
분류Animal Origin-Free
용액 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
HEPES BufferNo HEPES

구성 및 보관


  • Storage conditions: 2°C to 8°C (protect from light)
  • Shipping conditions: Ambient
  • Shelf life: 12 months from date of manufacture

배지 조성


  • 11875 - RPMI 1640