Pierce™ BCA Protein Assay Kit 

Product Number : 23227


Pierce™ BCA Protein Assay Kit 

Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.


Features of the BCA Protein Assay Kit include:

  • Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
  • Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
  • Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
  • Assay time—30-min incubation; much easier and four times faster than classical Lowry methods
  • Assay range—linear working range for BSA of 20 to 2000 µg/mL
  • Sensitivity—detect down to 5 µg/mL with enhanced protocol


How the assay works

The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.


In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.


The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods, the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.


사양


설명Pierce BCA Protein Assay Kit
어세이Protein Quantitation Assay
검출 방법Colorimetric
제품라인Pierce™
특이성Not Target-Specific
용도(애플리케이션)Solution-based Detection, Absorbance
용도(장비)Spectrophotometer, Microplate Reader
수량500 mL Kit

구성 및 보관


Sufficient For: 250 tube assays or 2500 microplate assays

  • BCA Reagent A, 500 mL
  • BCA Reagent B, 25 mL
  • Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL


Store at room temperature


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주식회사 에스앤씨  |  SNC CO., LTD.

사업자번호. 126-86-37254   |   대표. 성진재

소재지. 경기 하남시 조정대로 150 아이테코 1019호  [지도보기]

전화. 1644 5778  |  팩스. 031 790 1790

홈페이지. www.sncbio.co.kr  |  고객지원센터

개인정보보호책임자. 사업개발부/온라인사업팀

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