TransIT-2020 Transfection Reagent

Product Number : MIR5400


TransIT-2020 Transfection Reagent

TransIT®-2020은 광범위한 mammalian cell에 뛰어난 효율로 DNA plasmid를 도입하는 제품이다.


 TransIT-2020®은 유전자 도입이 어려운 세포의 일시적인 (transient) 유전자 도입과 영구적인 (stable) 유전자 도입에 적합하다.


TransIT-2020®은 동물 유래 성분이 포함하지 않으며, serum이 포함된 배지에 사용 가능하기 때문에, transfection 이후 번거로운 배지 교체 작업이 필요 없이 간편하게 사용 할 수 있다.


Figure 1. TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents.

Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.




Figure 2. High Performance Plasmid Transfection.

Primary Human Small Epithelial Cells (HSAEpic) were transfected using  TransIT®-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.




Figure 3. Superior Gene Expression in a Broad Spectrum of Cell Types.

The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1 μg/well) according to industry accepted testing protocols. Reagent to DNA ratios were optimized for each cell type:  TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine® 2000 (Life Technologies, 1.5:1, 3:1 or 5:1).  Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of luciferase expression. FuGENE® is a registered trademark of Fugent LLC. Lipofectamine® is a trademark of Life Technologies Corporation.

 


Mirus Best3 비교 확인


특징
TransIT-X2® Dynamic
Delivery System
(Code MIR 6000)

TransIT®-LT1

Transfection Reagent

(Code MIR 2300)

TransIT®-2020

 Transfection Reagent

(Code MIR 5400)

도입 물질
DNA, siRNA,
miRNA, RNP

DNA only
(Plasmid)
DNA only
(Plasmid)
세포 독성
Minimal Toxicity
Exceptionally
Low Toxicity

Minimal Toxicity
Animal Origin Free
O

X

O
Application

CRISPR/Cas9

Co-Transfection

iPSC

독성에 민감한Primary cell

Hard to Transfection cell에 최적
선택 포인트
하나의 제품으로다양한 실험에 적용할 때
독성에 민감한 세포에 Transfection을 할 때
강력한 유전자 도입 효율이 필요할 때

보존 4℃


주식회사 에스앤씨  |  SNC CO., LTD.

사업자번호. 126-86-37254   |   통신판매업 신고번호 : 제2022-경기하남-2257호   |   대표. 성진재   |   소재지. 경기 하남시 조정대로 150(아이테코), 10층 1017, 1019호  [지도보기]

전화. 1644 5778  |  팩스. 031 790 1790  |  홈페이지. www.sncbio.co.kr   |   개인정보보호책임자. 사업개발부/온라인사업팀

이용약관  |  개인정보처리방침  |  사업자정보확인  |  취급 브랜드 확인하기



주식회사 에스앤씨  |  SNC CO., LTD.

사업자번호. 126-86-37254   |   대표. 성진재

소재지. 경기 하남시 조정대로 150 아이테코 1019호  [지도보기]

전화. 1644 5778  |  팩스. 031 790 1790

홈페이지. www.sncbio.co.kr  |  고객지원센터

개인정보보호책임자. 사업개발부/온라인사업팀

이용약관  |  개인정보처리방침


SNC SOCIAL NETWORKS